Standard safety practices in the microbiology laboratory. Microbes in action activities are not pathogenic for humans. Savannah thompson august 22, 2014 microbiology lab friday section 3 ten lab safety rules 1. Amarillo college microbiology laboratory safety rules. This procedure applies to all employees and students at tru. Safety in the microbiology laboratory biology libretexts. Wash your hands with soap and water before entering or leaving the lab. The laboratory safety manual is intended to address universal safety measures for achieving a safe and healthy working environment. You must wear a lab coat when working in the microbiology lab.
Preventing labacquired infections bacteria viruses fungi human blood, unfixed tissue human cell lines recombinant dnadr. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. Shop carolina s variety of lab equipment including microscopes, glassware, dissection supplies, lab furniture and more. Rules and safety in microbiology laboratory youtube. Ppt introduction to microbiology and laboratory safety. The guidelines are straightforward and largely common. Some of these rules and procedures are listed in the beginning material of your laboratory manual. The mlg contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. Microbiology laboratory safety and rules biology ii mrs. The lab safety plan will provide more detailed safety information for the hazards present in the lab.
Laboratory personnel must receive training regarding the laboratory standard, the chp, and other laboratory safety practices, including exposure detection, physical and health hazards associated with chemicals, and protective measures. There are several causes of laboratory accidents each year. Bagleys office 4872385 microbiology teaching lab 4872458 biological sciences office 4872025 outside emergency agencies 8911 16. Alright, now in this part of the article, you will be able to access the free pdf download of microbiology. Rules and safety measures in microbiological laboratory. Safety procedures and precautions in the microbiology. Learn vocabulary, terms, and more with flashcards, games, and other study tools. It is very important to follows rules of microbiological laboratory in safety point of view. It describes good laboratory practices that must be understood and observed by all individuals involved in the laboratory. Although the organisms used in all science in the real world. Hazards exist in the lab for toxic chemicals and microorganisms. These include using model risk assessments for which it is necessary to refer to appropriate publications such as cleapss laboratory handbook 2006, section 15.
A free powerpoint ppt presentation displayed as a flash slide show on id. If these important rules are not followed a student,teacher, or a laboratory worker can face biohazards, chemical hazards as well as physical hazards. If you need help, dial 123 on the lab phone to get the campus emergency office. Safety at work act to comply with any safety instructions given by their employers.
Asm guidelines for biosafety in teaching laboratories. Bsl2 work is performed with bacteria and viruses that cause only mild disease to humans, or are difficult to contract via aerosol in a lab setting. The three main elements of safe containment of microorganisms are 1 good laboratory practices and technique, 2 safety equipment, and 3 facility design. For the safety of lab personnel, hazardous waste must be collected and transported by professionals. Microbiology safety guidelines page 1 of 25 usm0021 11052012 rev 01. These include using model risk assessments for which it is necessary to refer to appropriate publications such as cleapps laboratory handbook 2006, section 15. Upon completion of the lecture, accompanying video, required readings and clinical rotations, the student will be able to. Microbes, in particular have a great versatilty to grow and proliferate in different environments i. In the following phase, we designed a laboratory notebook, brochures, safety signs, safety information cards, etc. Biosafety in microbiological and biomedical laboratories bmbl quickly became the cornerstone of biosafety practice and policy in the united states upon first publication in 1984. The laboratory is a place for serious work and not a place to play. Think about your own safety and that of others working around you. Safety in microbiology laboratory pdf safety in the microbiology laboratory. This faculty specific laboratory safety handbook has been prepared as a result of these extensive efforts.
Safety in a microbiology laboratory is important in the prevention of infection as microbiology laboratory cultures, manipulates, and uses virulent andor potentially pathogenic microorganisms. In addition to those rules and procedures listed in the back of your laboratory manual, other important rules are listed below. Choose from 500 different sets of 1 microbiology lab safety flashcards on quizlet. After being signed please put this entire packet in the instructors desk look for an envelope with your name. Limiting access to laboratory sometimes, people who do not work in the laboratory attempt to enter the laboratory to look for test results they desire. Locate and properly use basic emergency equipment such as eye wash stations, first aid kits, fire extinguishers, a telephone, etc. Use the following list of guidelines for proper disposal. If these important rules are not followed a student,teacher, or a laboratory worker can face biohazards, chemical hazards as. To ensure safer downloads, we have uploaded this pdf ebook file to our online file repository. Wear safety glasses when handling bacterial broth cultures, doing gram stains, and as otherwise advised by your instructor. Most municipalities have specific policies for lab safety and waste disposal, so check with local government officials to understand and comply with any regulations. The biosafety guidelines are for teaching labs that use only bsl1 and bsl2 organisms. Lake worth laboratory safety rules verification form. The asm biosafety guidelines provide educators with a consistent way to safely work with microorganisms in the classroom and to further prevent the spread of microorganisms.
No eating, drinking, smoking or chewing gum is allowed in the laboratory. The three most common are, 1 inappropriate use of chemicals and equipment, 2 inexperienced students. Lake worth laboratory safety rules verification form microbiology mcb 2010l professors, please have the students read and sign the document attached. Microbiology lab safety university of minnesota duluth. For a quarter century, carolina biological supply has been committed to bringing biotechnology into the classroom. The safe handling of microorganisms in the teaching laboratory is a top priority. Laboratory safety manual the laboratory safety manual is intended to address universal safety measures for achieving a safe and healthy working environment. All accidents, cuts, and any damaged glassware or equipment should be reported to the lab instructor immediately. Microbiology laboratory safety rules and procedures youngstown state university each student enrolled in a microbiology laboratory at youngstown state university must follow specific safety rules and procedures. Do not engage in rowdy, playful, or unprofessional activities in the laboratory.
Introduction to microbiology and laboratory safety biosafety for micr 2909 lecture 2, 2001 bscmolbiol lect 2. The microbiology laboratory guidebook mlg contains test methods used by the fsis field service laboratories to support fsis regulatory activities. Laboratory safety is governed by numerous local, state and federal regulations. Historically, the information in this publication has been advisory is nature even though legislation and regulation, in some circumstances, have overtaken it and made compliance with the guidance provided mandatory. In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. The athabasca university microbiology lab safety rules. Wear shoes that completely cover your feet, you cannot wear sandals or other opentoe or openheel shoes clogs are ok. The guidelines contain requirements on these topics. I reduce or eliminate exposure of lab workers, other persons, and the outside environment to potentially hazardous microorganisms. Browse the latest lab supplies and equipment for all your science lab essentials. Failure to follow these safety rules may result in loss of points from the related assignments. A laboratory manual pdf using our direct download link mentioned at the end of this article. Microbiology laboratory safety rules never work alone in the laboratory without permission and prior knowledge of the instructor. Microbiology laboratory safety rules and procedures.
Safety information safety guidelines microbiology online. Biosafety in microbiology laboratory linkedin slideshare. General safety rules and procedures the laboratory procedures must be read prior to attending that laboratory session. Microbiology laboratory safety rules and procedures each student enrolled in a microbiology laboratory at youngstown state university must follow specific safety rules and procedures. Microbiology lab safety printed copies of this document are uncontrolled. It is the responsibility of the student to know the location and use of all safety equipment in the lab eyewash, fire extinguisher, etc. Wash your hands before and after conducting an experiment. Failure to follow safety rules may result in removal from the class. Keep hands away from your face, eyes, and mouth when working with chemicals or microorganisms. To ensure the safety of people in the microbiology laboratories at thompson rivers university tru.
This document is intended for supervisors, principal investigators and managers who have the primary responsibility for maintaining laboratories under. Biosafety in microbiological and biomedical laboratories. Lake worth laboratory safety rules verification form microbiology. Knowledge of safety rules and equipment is the first component of laboratory safety. Wear proper attire for the microbiology lab at all times lab coat that comes down to. It can also result in the removal from class on the day of the. Objectives students successfully completing this laboratory exercise should be able to. Standard microbiological safety practices the following safety guidelines listed below apply to all microbiology laboratories, regardless of biosafety level.
Laboratory safety rules these rules are for the safety of the students, instructors and support staff. Laboratory safety adapted from the american society of microbiology website. Learn 1 microbiology lab safety with free interactive flashcards. Nine safe practices for the microbiology laboratory. Sep 25, 2019 bsl1 lab is used to perform research mostly on noninfectious microbes using standard equipment and routine lab safety procedures. Biosafety guidelines appendix one of the many hats that educators must wear is to prevent the inadvertent passage of microbes out of the teaching laboratory by a student or assistant. Laboratory safety guidance occupational safety and.
Oct 16, 2017 rules and safety measures in microbiological laboratory it is very important to follows rules of microbiological laboratory in safety point of view. Microbiology laboratory safety rules and procedure learn. Safety procedures and precautions in the microbiology laboratory all safety procedures and precautions followed in the microbiology laboratory are designed to. Over the years, osha has promulgated rules and published guidance to make laboratories increasingly safe for personnel. Microbiology laboratory safety rules the following procedures should be observed at all times to ensure safety in the laboratory. Emmett barkley, phd director, office of laboratory safety howard hughes medical institute chevy chase, md, usa 1 safety in healthcare laboratories. Wear lab aprons or lab coats as advised by your instructor.
1024 1512 1571 78 1365 229 728 973 7 434 1078 1300 1049 1251 527 464 382 1394 349 1241 1061 418 292 865 1260 273 1588 15 1160 497 448 1090 209 177 161 952 572 895 1137 578 475 257 39